Sybr Green Qpcr

With the development of thermal cyclers incorporating fluorescent detection pcr has a new innovative application.

Sybr green qpcr.

Standard curve r 2 0 999 β actin efficiency 92 6 slope. Direct detection of pcr product is monitored by measuring the increase in. It is used in quantitative pcr because the fluorescence can be measured at the end of each amplification cycle to determine relatively or absolutely how much dna has been amplified. Applied biosystems sybr green master mixes are designed for quantitative real time pcr using a set of two pcr primers that flank the target region.

Itaq universal sybr green supermix produced greater than 90 efficiency over 6 orders of linear dynamic range. Itaq universal sybr green supermix provides reliable gene expression data. The kit is based on the proven technology of quantitect sybr green kits. The master mixes contain buffer dntps thermostable hot start dna polymerase and sybr green dye everything needed for real time pcr.

The quantifast sybr green rt pcr kit delivers highly specific and sensitive results outperforming other real time rt pcr kits used in fast cycling mode see figure specific one step rt pcr rt pcr run times are reduced by up to 60 see figure significantly reduced pcr times allowing you to get results faster you can also greatly increase your sample throughput or efficiently share a. Sybr green i is a commonly used fluorescent dye that binds double stranded dna molecules by intercalating between the dna bases. Sybr green for qpcr. The human β actin gene was amplified from hela cdna 100 ng 100 fg using a cfx96 real time pcr detection system.

Sybr green qpcr supermixes. Sybr green pcr master mix and sybr green rt pcr reagents kit user guide 9 1 product information purpose of the kit the sybr green pcr master mix is a convenient premix of the components except primers template and water necessary to perform real time pcr using sybr green i dye. Cdna generated by the miscript ii rt kit is used as the template for real time pcr with the miscript sybr green pcr kit and either pcr arrays mirnome or pathway focused miscript mirna pcr arrays or. Sybr green qpcr supermixes are compatible with most protocols for qpcr.

Find the best master mix by application. The miscript sybr green pcr kit is an integral component of the miscript pcr system for mirna detection and quantification. In addition sybr green qpcr supermixes are available with either antibody mediated hot start taq or sso7d fusion polymerase which provides increased stability for the polymerase template complex enhancing performance. In routine pcr the critical result is the final quantity of amplicon generated after the process.

Applied biosystems amplitaq gold dna polymerase ld is a hot start pcr enzyme provided in an inactive state allowing for room temperature set up and minimizing non specific amplification.