Sybr Green Master Mix

The master mixes contain buffer dntps thermostable hot start dna polymerase and sybr green dye everything needed for real time pcr.

Sybr green master mix.

It contains faststart taq dna polymerase and dna double strand specific sybr green i dye for product detection and characterization. Power sybr green pcr master mix contains all of the components excluding the template and primers for superior sybr green reagent based real time pcr. The lightcycler 480 sybr green i master is a one component hot start reaction mix for pcr. Faststart universal sybr green master rox is a ready to use hot start reaction mix for qpcr and rt qpcr on all real time pcr systems requiring normalization with rox.

As the pcr reaction proceeds at each round of amplification sybr green dye binds to dsdna as it polymerizes resulting in an increase in the level of fluorescence at the end of each extension step. Find the best master mix by application. Sybr green dye is a fluorescent double stranded dna dsdna binding dye that is used to track the progress of dna amplification in real time pcr experiments. Sufficient reagents provided for 40 reactions based on a 50 µl reaction volume.

Power sybr green pcr master mix provides greater flexibility reduces experimental design time and requires fewer reagents. Sybr green pcr master mix in a 1 ml vial. Powerup sybr green master mix contains proprietary dual lock taq dna polymerase enzyme which uses a combination of two hot start mechanisms to control its activity. Coupled with user supplied primer sets and template powerup sybr green master mix is designed to amplifiy targets for accurate gene expression analysis.

The azuraquant green fast qpcr mix is a ready to use 2x master mix for use in real time quantitative pcr assays in which intercalating dye based detection provides the option of a post amplification melt profile. Applied biosystems amplitaq gold dna polymerase ld is a hot. Providing tight control over taq activation this dual hot start approach helps prevent undesirable early activity of the polymerase at low temperatures that can lead to non specific amplification. Sybr green master mix advantages.

General description faststart universal sybr green master rox. Applied biosystems sybr green master mixes are designed for quantitative real time pcr using a set of two pcr primers that flank the target region. Powerup sybr green master mix is a pre formulated optimized universal 2x master mix for real time pcr workflows. Guaranteed minimum shelf life is 60 days exact expiry date printed on product and cofa.