Sybr Green Gel Stain

Sybr green i is also a very sensitive stain for oligonucleotides allowing for detection of as little as 1 2 ng of a synthetic 24 mer on a 5 polyacrylamide gel which is 50 100 times greater sensitivity than obtained with ethidium bromide.

Sybr green gel stain.

Sybr safe dna gel stain does not differ significantly from 0 5x tbe buffer when tested according to national pollutant discharge elimination system npdes guidelines table 2. Lonza sybr green i nucleic acid gel stain for the detection of dsdna and oligonucleotides in agarose and polyacrylamide gels 446 50 781 50. Sybr green i nucleic acid gel stain is one of the most sensitive stains available for detecting double stranded dna dsdna in agarose and polyacrylamide gels. Sybr green i stain has been shown to be much less mutagenic than ethidium bromide in ames tests.

When bound to nucleic acids the green fluorescent sybr safe stain has fluorescence excitation maxima at 280 and 502 nm and an emission maximum at 530 nm see figure. Because sybr green i has greater sensitivity for dsdna it is especially useful for assays where the presence of contaminating rna or ssdna might obscure results. Plus when used with blue light illumination sybr safe stain has less background fluorescence than ethidium bromide stained gels illuminated with uv light.